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e6 1 atcc tib 152 nctc  (ATCC)


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    ATCC e6 1 atcc tib 152 nctc
    E6 1 Atcc Tib 152 Nctc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e6 1 atcc tib 152 nctc  (ATCC)
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    cell lines mouse raw264 7 cell atcc  (ATCC)
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    Anti-inflammatory and antioxidative capacity of hydrogels. Flow cytometry (A) reveals that IBU-CS-GP hydrogel significantly promote M2 polarization of LPS-stimulated RAW264.7 <t>macrophages</t> (B) RT-PCR analysis suggests that IBU-CS-GP downregulates pro-inflammatory gene TNF-α (C) and upregulates anti-inflammatory genes of IL-4 (D) in comparison to CS-MA and CS-GP. IBU-CS-GP markedly attenuates ROS and NO production in LPS-stimulated macrophages, as indicated by DCFH staining (H), flow cytometry analysis (G), and ROS & NO quantification (E–F). One-way ANOVA with Tukey's post hoc test and n = 3 for B-F; ∗∗p < 0.01, ∗∗∗p < 0.001, n.s. ANOVA, analysis of variance; CS, chitosan; IBU, ibuprofen; GP, genipin; MA, methacrylic anhydride; LPS, lipopolysaccharides; n.s., not significant; NO, nitric oxide; PCR, polymerase chain reaction; ROS, reactive oxygen species; DCFH, 2′,7′-Dichlorodihydrofluorescein; DAPI, 4′,6-diamidino-2-phenylindole.
    Cell Lines Mouse Raw264 7 Cell Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    atcc atcc tib  (ATCC)
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    Anti-inflammatory and antioxidative capacity of hydrogels. Flow cytometry (A) reveals that IBU-CS-GP hydrogel significantly promote M2 polarization of LPS-stimulated RAW264.7 <t>macrophages</t> (B) RT-PCR analysis suggests that IBU-CS-GP downregulates pro-inflammatory gene TNF-α (C) and upregulates anti-inflammatory genes of IL-4 (D) in comparison to CS-MA and CS-GP. IBU-CS-GP markedly attenuates ROS and NO production in LPS-stimulated macrophages, as indicated by DCFH staining (H), flow cytometry analysis (G), and ROS & NO quantification (E–F). One-way ANOVA with Tukey's post hoc test and n = 3 for B-F; ∗∗p < 0.01, ∗∗∗p < 0.001, n.s. ANOVA, analysis of variance; CS, chitosan; IBU, ibuprofen; GP, genipin; MA, methacrylic anhydride; LPS, lipopolysaccharides; n.s., not significant; NO, nitric oxide; PCR, polymerase chain reaction; ROS, reactive oxygen species; DCFH, 2′,7′-Dichlorodihydrofluorescein; DAPI, 4′,6-diamidino-2-phenylindole.
    Atcc Atcc Tib, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cell lines raw264 7 american type culture collection tib  (ATCC)
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    Anti-inflammatory and antioxidative capacity of hydrogels. Flow cytometry (A) reveals that IBU-CS-GP hydrogel significantly promote M2 polarization of LPS-stimulated RAW264.7 <t>macrophages</t> (B) RT-PCR analysis suggests that IBU-CS-GP downregulates pro-inflammatory gene TNF-α (C) and upregulates anti-inflammatory genes of IL-4 (D) in comparison to CS-MA and CS-GP. IBU-CS-GP markedly attenuates ROS and NO production in LPS-stimulated macrophages, as indicated by DCFH staining (H), flow cytometry analysis (G), and ROS & NO quantification (E–F). One-way ANOVA with Tukey's post hoc test and n = 3 for B-F; ∗∗p < 0.01, ∗∗∗p < 0.001, n.s. ANOVA, analysis of variance; CS, chitosan; IBU, ibuprofen; GP, genipin; MA, methacrylic anhydride; LPS, lipopolysaccharides; n.s., not significant; NO, nitric oxide; PCR, polymerase chain reaction; ROS, reactive oxygen species; DCFH, 2′,7′-Dichlorodihydrofluorescein; DAPI, 4′,6-diamidino-2-phenylindole.
    Cell Lines Raw264 7 American Type Culture Collection Tib, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Anti-inflammatory and antioxidative capacity of hydrogels. Flow cytometry (A) reveals that IBU-CS-GP hydrogel significantly promote M2 polarization of LPS-stimulated RAW264.7 macrophages (B) RT-PCR analysis suggests that IBU-CS-GP downregulates pro-inflammatory gene TNF-α (C) and upregulates anti-inflammatory genes of IL-4 (D) in comparison to CS-MA and CS-GP. IBU-CS-GP markedly attenuates ROS and NO production in LPS-stimulated macrophages, as indicated by DCFH staining (H), flow cytometry analysis (G), and ROS & NO quantification (E–F). One-way ANOVA with Tukey's post hoc test and n = 3 for B-F; ∗∗p < 0.01, ∗∗∗p < 0.001, n.s. ANOVA, analysis of variance; CS, chitosan; IBU, ibuprofen; GP, genipin; MA, methacrylic anhydride; LPS, lipopolysaccharides; n.s., not significant; NO, nitric oxide; PCR, polymerase chain reaction; ROS, reactive oxygen species; DCFH, 2′,7′-Dichlorodihydrofluorescein; DAPI, 4′,6-diamidino-2-phenylindole.

    Journal: Materials Today Bio

    Article Title: Injectable chitosan-based hydrogel via in situ gelation modulates the inflammatory microenvironment and facilitates minimally invasive repair of peripheral nerve injury

    doi: 10.1016/j.mtbio.2026.102814

    Figure Lengend Snippet: Anti-inflammatory and antioxidative capacity of hydrogels. Flow cytometry (A) reveals that IBU-CS-GP hydrogel significantly promote M2 polarization of LPS-stimulated RAW264.7 macrophages (B) RT-PCR analysis suggests that IBU-CS-GP downregulates pro-inflammatory gene TNF-α (C) and upregulates anti-inflammatory genes of IL-4 (D) in comparison to CS-MA and CS-GP. IBU-CS-GP markedly attenuates ROS and NO production in LPS-stimulated macrophages, as indicated by DCFH staining (H), flow cytometry analysis (G), and ROS & NO quantification (E–F). One-way ANOVA with Tukey's post hoc test and n = 3 for B-F; ∗∗p < 0.01, ∗∗∗p < 0.001, n.s. ANOVA, analysis of variance; CS, chitosan; IBU, ibuprofen; GP, genipin; MA, methacrylic anhydride; LPS, lipopolysaccharides; n.s., not significant; NO, nitric oxide; PCR, polymerase chain reaction; ROS, reactive oxygen species; DCFH, 2′,7′-Dichlorodihydrofluorescein; DAPI, 4′,6-diamidino-2-phenylindole.

    Article Snippet: Mouse fibroblasts (L929, ATCC), human umbilical vein endothelial cells (HUVECs, ATCC), and mouse macrophages (RAW 264.7, ATCC) were provided by the Cell Bank of the Chinese Academy of Sciences.

    Techniques: Flow Cytometry, Reverse Transcription Polymerase Chain Reaction, Comparison, Staining, Polymerase Chain Reaction

    Pro-angiogenic and pro-migratory effects of hydrogels. Immunofluorescence staining shows blood vessel formation of HUVECs in LPS-macrophage condition medium (A). Scratch assays and Transwell migration assays of HUVECs (B) and L929 cells (C). Quantification of junctions (D), branches (E), wound closure percentage (F–G) and migrated cells (H–I). One-way ANOVA with Tukey's post hoc test and n = 3 for D-I; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, n.s. ANOVA, analysis of variance; CS, chitosan; IBU, ibuprofen; GP, genipin; MA, methacrylic anhydride; LPS, lipopolysaccharides; HUVEC, human umbilical vein endothelial cell; n.s., not significant.

    Journal: Materials Today Bio

    Article Title: Injectable chitosan-based hydrogel via in situ gelation modulates the inflammatory microenvironment and facilitates minimally invasive repair of peripheral nerve injury

    doi: 10.1016/j.mtbio.2026.102814

    Figure Lengend Snippet: Pro-angiogenic and pro-migratory effects of hydrogels. Immunofluorescence staining shows blood vessel formation of HUVECs in LPS-macrophage condition medium (A). Scratch assays and Transwell migration assays of HUVECs (B) and L929 cells (C). Quantification of junctions (D), branches (E), wound closure percentage (F–G) and migrated cells (H–I). One-way ANOVA with Tukey's post hoc test and n = 3 for D-I; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, n.s. ANOVA, analysis of variance; CS, chitosan; IBU, ibuprofen; GP, genipin; MA, methacrylic anhydride; LPS, lipopolysaccharides; HUVEC, human umbilical vein endothelial cell; n.s., not significant.

    Article Snippet: Mouse fibroblasts (L929, ATCC), human umbilical vein endothelial cells (HUVECs, ATCC), and mouse macrophages (RAW 264.7, ATCC) were provided by the Cell Bank of the Chinese Academy of Sciences.

    Techniques: Immunofluorescence, Staining, Migration